THE PRESENCE OF CD30 ON T CELLS IN THE INFLAMMATORY INFILTRATE OF ACUTE ATOPIC DERMATITIS

Autori:

DUBRAVKA BOBEK, JASNA LIPOZENČIĆ, JASMINKA JAKIĆ-RAZUMOVIĆ, DAVOR IVANKOVIĆ, OLGA BADOVINAC

Sažetak
Atopijski dermatitis (AD) sadržava stanične imunohistokemijske značajke slične onima kod kontaktnog alergijskog dermatitisa (KAD). Postoji obilje dokaza za T-staničnu aktivaciju u AD-u kao što su nazočnost T-limfocita koji nose biljege CD3, CD4 i CD45RO. Cilj ovog rada bio je prikazati pojavnost površinskih limfocitnih antigena uključujući CD30, CD45RO, CD4 i CD3 u bolesnika s akutnim AD-om te usporediti pojavnost tih molekula u bolesnika s akutnim KAD-om na nikal radi mogućeg morfološkog razlikovanja tih dvaju entiteta. Pojavnost navedenih molekula evaluirana je imunohistokemijski u bioptatima ledirane kože u dermisu i epidermisu. Biopsije su uzete od 12 pacijenata koji su bolovali od akutnog AD i od 13 pacijenata s KAD-om. Rezultati pokazuju statistički značajno viši prosjek pojavnosti, a također bitno veći raspon pojavnosti limfocita CD30+, CD45RO+, CD3+ i CD4+ u dermisu i epidermisu bolesnika s AD-om, u odnosu na prosjek i raspon pojavnosti u bolesnika s KAD-om. Pri tome uočena je statistički značajno veća pojavnost CD30, CD45RO, CD3 i CD4 u dermisu nego u epidermisu u obje skupine bolesnika. Povišeni broj CD30+ limfocita u bolesnika s akutnim AD-om, međutim, ne korelira s težinom kliničke slike procijenjene po kliničkom skoru za evaluaciju težine bolesti, tzv. SCORAD-indeksu (engl. Severity Scoring of Atopic Dermatitis). Naši rezultati pokazuju povezanost ekspresije CD30 i akutne faze AD-a, ali ne i KAD-a, što bi moglo utvrditi CD30 kao koristan biljeg u diferenciranju tih dviju bolesti.
Summary

Summary. Atopic dermatitis (AD) has cellular immunohistochemical features similar to those of allergic contact dermatitis (ACD). There is plenty of evidence for T-cell activation in this disease such us the presence of T-lymphocytes which carry CD30, CD3, CD4, CD45RO markers. The aim of this study was to show the presence of lymphocyte-surface antigens including CD30, CD3, CD4, CD45RO in patients with acute AD and to compare the presence of the same molecules in patients with acute ACD (nickel-induced) because of the possible morphologic difference of these two entities. The presence of the stated molecules is immunohistochemically evaluated in biopsies of lesional skin in dermis and epidermis. Biopsies were obtained from twelve patients suffering from acute AD and from thirteen patients with ACD. The results show statistically significant higher average of presence and also much higher range of presence of CD30+, CD3+, CD4+, CD45RO+ lympohocytes in dermis and epidermis of patients with AD compared to the average and range in patients with ACD. Statistically much higher average of CD30, CD3, CD4, CD45RO is noticed in those occasions more in dermis than in epidermis in both groups of patients. High number of CD30+ lymphocytes in patients with acute AD does not however correlate with the severity of the disease evaluated according to clinical score for the evaluation of the severity of the disease, so called the SCORAD-index (Severity Scoring of Atopic Dermatitis). Our results showed an association betwen CD30 expression and acute AD but not with acute ACD which could evaluate CD3 as the useful marker in differentiating these two diseases.

Volumen: 11-12, 2004

Liječ Vjesn 2004;126:291–297

Davor Ivankovic, Dubravka Bobek, Jasminka Jakić-Razumović, Jasna Lipozenčić, OLGA BADOVINAC

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